ENHANCEMENT IN CELLULASE PRODUCTION BY TRICHODERMA-REESEI RUT-C30 DUE TO CITRIC-ACID

被引：14

作者：

KADAM, KL

KEUTZER, WJ

机构：

[1] Alternative Fuels Division, National Renewable Energy Laboratory, Golden, 80401, CO

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 10期

关键词：

D O I：

10.1007/BF00143112

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This report discusses enhancement in cellulase production by Trichoderma reesei Rut-C30. A combination of 75 mM citrate buffer and low operating pH values; e.g., 3.5, resulted in about 100% higher enzyme production in shake flasks. A similar extent of improvement was seen at lower citrate buffer concentrations such as 5 and 10 mM. It is suggested that the undissociated form of citric acid is responsible for this improvement; no such acid moiety has been previously reported to enhance cellulase production.

引用

页码：1111 / 1114

页数：4

共 9 条

[1] CELLULASE PRODUCTION INDUCED BY CARBON-SOURCES DERIVED FROM WASTE NEWSPAPER
CHEN, S
WAYMAN, M
[J]. PROCESS BIOCHEMISTRY, 1991, 26 (02) : 93 - 100
[2] MEASUREMENT OF CELLULASE ACTIVITIES
GHOSE, TK
[J]. PURE AND APPLIED CHEMISTRY, 1987, 59 (02) : 257 - 268
[3] KAWAMORI M, 1986, APPL MICROBIOL BIOT, V24, P449
[4] THE TRICHODERMA CELLULASE REGULATORY PUZZLE - FROM THE INTERIOR LIFE OF A SECRETORY FUNGUS
KUBICEK, CP
MESSNER, R
GRUBER, F
MACH, RL
KUBICEKPRANZ, EM
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1993, 15 (02) : 90 - 99
[5] USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR
MILLER, GL
[J]. ANALYTICAL CHEMISTRY, 1959, 31 (03) : 426 - 428
[6] POMPEI R, 1991, BIOTECHNIQUES, V11, P703
[7] SAHOO DK, 1986, J GEN MICROBIOL, V132, P2761
[8] ENHANCED PRODUCTION OF CELLULASE, HEMICELLULASE, AND BETA-GLUCOSIDASE BY TRICHODERMA-REESEI (RUT C-30)
TANGNU, SK
BLANCH, HW
WILKE, CR
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1981, 23 (08) : 1837 - 1849
[9] WILEY DF, 1985, THESIS U CALIFORNIA

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