SUSCEPTIBILITY OF LEUCROSE TO CARBOHYDRASES

被引:6
作者
IIZUKA, M [1 ]
HIYAMA, M [1 ]
ITAYA, K [1 ]
FURUICHI, K [1 ]
ANN, YG [1 ]
MINAMIURA, N [1 ]
YAMAMOTO, T [1 ]
机构
[1] FUKUYAMA UNIV,FAC ENGN,DEPT BIOENGN,HIGASHIMURA,FUKUYAMA 72902,JAPAN
来源
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1990年 / 70卷 / 04期
关键词
D O I
10.1016/0922-338X(90)90064-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Oligosaccharides were synthesized from sucrose using the dextransucrase of Leuconostoc mesenteroides, and the products were isolated by chromatography. The oligosaccharide obtained as the main product was identified as leucrose by 13C-NMR. Production of leucrose from sucrose was greatly increased when the reaction was done with fructose as an acceptor. The susceptibility of leucrose to carbohydrases was tested, and leucrose was also a useful substrate for detecting or measuring α-glucosidase in enzyme mixtures such as culture filtrates of microorganisms. © 1990.
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页码:277 / 279
页数:3
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