IDENTIFICATION AND LOCALIZATION OF A SOLUBLE-ANTIGEN, AG2, OF 136-KDA FROM PLASMODIUM-FALCIPARUM INVITRO CULTURES

被引：4

作者：

JAKOBSEN, PH

GRELLIER, P

THEANDER, TG

BEHRENDT, N

TORII, M

AIKAWA, M

SCHREVEL, J

JEPSEN, S

机构：

[1] UNIV POITIERS,CNRS 290,BIOL CELLULAIRE LAB,F-86022 POITIERS,FRANCE

[2] RIGSHOSP,DEPT INFECT DIS,LYMPHOCYTE LAB,DK-2100 COPENHAGEN,DENMARK

[3] RIGSHOSP,FINSEN LAB,DK-2100 COPENHAGEN,DENMARK

[4] CASE WESTERN RESERVE UNIV,INST PATHOL,CLEVELAND,OH 44106

[5] STATENS SERUM INST,WHO,NATL MALARIA DIAGNOST CTR,DK-2300 COPENHAGEN,DENMARK

来源：

APMIS | 1991年 / 99卷 / 02期

关键词：

MALARIA; PLASMODIUM-FALCIPARUM; SOLUBLE ANTIGENS; FPLC;

D O I：

10.1111/j.1699-0463.1991.tb05133.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X-100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.

引用

页码：155 / 162

页数：8

共 22 条

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BALLET JJ, 1986, ACTA TROP, V43, P255

[2]

BALLET JJ, 1982, INFECT IMMUN, V33, P758

[3] PLASMODIUM-FALCIPARUM - MOLECULAR ANALYSIS OF A PUTATIVE PROTECTIVE ANTIGEN, THE THERMOSTABLE 96-KDA PROTEIN [J].