CHANGES IN FIBRONECTIN MESSENGER-RNA SPLICING WITH INVITRO PASSAGE

被引：17

作者：

BURKE, EM ^{[1
]}

DANNER, DB ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV, DEPT MED, DIV GERIATR, BALTIMORE, MD 21224 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)90153-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Senescent human fibroblasts produce larger fibronectin molecules with altered binding properties. To determine if this change could involve alternative splicing of fibronectin precursor mRNA, we developed an approach using reverse transcription and the polymerase chain reaction to study fibronectin mRNA splicing at each of the three alternatively spliced regions. Two of the three regions showed changes with in vitro passage; incorporation of the ED-A region increased 8 fold. © 1991.

引用

页码：620 / 624

页数：5

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