STABILIZATION OF A YEAST ARTIFICIAL CHROMOSOME CONTAINING PLANT DNA USING A RECOMBINATION-DEFICIENT HOST

被引：14

作者：

DUNFORD, R ^{[1
]}

VILAGELIU, L ^{[1
]}

MOORE, G ^{[1
]}

机构：

[1] UNIV BARCELONA,FAC BIOL,DEPT GENET,E-08071 BARCELONA,SPAIN

来源：

PLANT MOLECULAR BIOLOGY | 1993年 / 21卷 / 06期

关键词：

YEAST ARTIFICIAL CHROMOSOME; INSTABILITY; RECOMBINATION-DEFICIENT HOST;

D O I：

10.1007/BF00023615

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The large genomes of many plant species contain numerous dispersed repeat sequences. The problem of large-scale structural instability due to the presence of such repeats in yeast artificial chromosome (YAC) clones was assessed. The feasibility of stabilising plant sequences prone to rearrangement in YACs was demonstrated using a host yeast strain deficient in recombination.

引用

页码：1187 / 1189

页数：3

共 8 条

[1] BOEKE J, 1989, MOBILE DNA, P543
[2] TRANSFORMATION OF YEAST SPHEROPLASTS WITHOUT CELL-FUSION
BURGERS, PMJ
PERCIVAL, KJ
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 163 (02) : 391 - 397
[3] CONSTRUCTION OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING BARLEY DNA AND THE IDENTIFICATION OF CLONES CARRYING COPIES OF THE REPEATED ELEMENT BIS-1
DUNFORD, R
ROGNER, UC
[J]. HEREDITAS, 1991, 115 (02) : 133 - 138
[4] REPEATED SEQUENCE DNA RELATIONSHIPS IN 4 CEREAL GENOMES
FLAVELL, RB
RIMPAU, J
SMITH, DB
[J]. CHROMOSOMA, 1977, 63 (03) : 205 - 222
[5] BIS-1, A MAJOR COMPONENT OF THE CEREAL GENOME AND A TOOL FOR STUDYING GENOMIC ORGANIZATION
MOORE, G
CHEUNG, W
SCHWARZACHER, T
FLAVELL, R
[J]. GENOMICS, 1991, 10 (02) : 469 - 476
[6] A FAMILY OF RETROTRANSPOSONS AND ASSOCIATED GENOMIC VARIATION IN WHEAT
MOORE, G
LUCAS, H
BATTY, N
FLAVELL, R
[J]. GENOMICS, 1991, 10 (02) : 461 - 468
[7] STRUCTURAL INSTABILITY OF HUMAN TANDEMLY REPEATED DNA-SEQUENCES CLONED IN YEAST ARTIFICIAL CHROMOSOME VECTORS
NEIL, DL
VILLASANTE, A
FISHER, RB
VETRIE, D
COX, B
TYLERSMITH, C
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (06) : 1421 - 1428
[8] Vilageliu L., 1992, Techniques for the analysis of complex genomes., P93

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