ISOLATION AND CHARACTERIZATION OF THE LYSOZYME-ENCODING GENE FROM THE SILKWORM BOMBYX-MORI

被引：65

作者：

LEE, WJ ^{[1
]}

BREY, PT ^{[1
]}

机构：

[1] INST PASTEUR,UNITE ECOL SYST VECTORIELS,F-75724 PARIS 15,FRANCE

来源：

GENE | 1995年 / 161卷 / 02期

关键词：

INSECT IMMUNITY; GENE EXPRESSION; UP-REGULATION; DEFENSE; CDNA CLONING; SEQUENCE ANALYSIS; EVOLUTION;

D O I：

10.1016/0378-1119(95)00199-G

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have isolated and characterized a Bombyx mori (Bm) cDNA encoding a lysozyme (Lyz). A 90-bp DNA fragment was amplified by PCR using degenerate oligodeoxyribonucleotide primers derived from the known amino acid (aa) sequence of the Bm Lyz. These PCR fragments were used to screen a fat body cDNA library. A clone containing the complete lys cDNA (1294 bp) was isolated and completely sequenced. The deduced 137-aa sequence showed high homology with other chicken-type Lyz. Bm lys gene expression was constitutive in fat body, cuticular epidermal tissue and at a very low level in hemocytes. This gene expression was up-regulated in fat body, hemocytes and cuticular epidermal tissue following the injection of Gram(+) bacteria.

引用

页码：199 / 203

页数：5

共 26 条

[1]

AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303

[2] ANTIBACTERIAL PEPTIDES - KEY COMPONENTS NEEDED IN IMMUNITY [J].

BOMAN, HG .

CELL, 1991, 65 (02) :205-207

[3] ROLE OF THE INTEGUMENT IN INSECT IMMUNITY - EPICUTICULAR ABRASION AND INDUCTION OF CECROPIN SYNTHESIS IN CUTICULAR EPITHELIAL-CELLS [J].

BREY, PT ;

LEE, WJ ;

YAMAKAWA, M ;

KOIZUMI, Y ;

PERROT, S ;

FRANCOIS, M ;

ASHIDA, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (13) :6275-6279

[4] CLONING OF HUMAN LYSOZYME GENE AND EXPRESSION IN THE YEAST SACCHAROMYCES-CEREVISIAE [J].

CASTANON, MJ ;

SPEVAK, W ;

ADOLF, GR ;

CHLEBOWICZSLEDZIEWSKA, E ;

SLEDZIEWSKI, A .

GENE, 1988, 66 (02) :223-234

[5] RECENT ORIGIN OF THE P-LYSOZYME GENE IN MICE [J].

CORTOPASSI, GA ;

WILSON, AC .

NUCLEIC ACIDS RESEARCH, 1990, 18 (07) :1911-1911

[6] THE LYSOZYME LOCUS IN DROSOPHILA-MELANOGASTER - AN EXPANDED GENE FAMILY ADAPTED FOR EXPRESSION IN THE DIGESTIVE-TRACT [J].

DAFFRE, S ;

KYLSTEN, P ;

SAMAKOVLIS, C ;

HULTMARK, D .

MOLECULAR & GENERAL GENETICS, 1994, 242 (02) :152-162

[7] CDNA AND AMINO-ACID-SEQUENCES OF RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) LYSOZYMES AND THEIR IMPLICATIONS FOR THE EVOLUTION OF LYSOZYME AND LACTALBUMIN [J].

DAUTIGNY, A ;

PRAGER, EM ;

PHAMDINH, D ;

JOLLES, J ;

PAKDEL, F ;

GRINDE, B ;

JOLLES, P .

JOURNAL OF MOLECULAR EVOLUTION, 1991, 32 (02) :187-198

[8]

DICKINSON L, 1988, J BIOL CHEM, V263, P19424

[9] SOLUBLE PEPTIDOGLYCAN FRAGMENTS STIMULATE ANTIBACTERIAL PROTEIN-SYNTHESIS BY FAT-BODY FROM LARVAE OF MANDUCA-SEXTA [J].

DUNN, PE ;

DAI, W ;

KANOST, MR ;

GENG, CX .

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1985, 9 (03) :559-568

[10] AMINO-ACID AND CDNA SEQUENCES OF LYSOZYME FROM HYALOPHORA-CECROPIA [J].

ENGSTROM, A ;

XANTHOPOULOS, KG ;

BOMAN, HG ;

BENNICH, H .

EMBO JOURNAL, 1985, 4 (08) :2119-2122

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