MOLECULAR-CLONING OF THE MOUSE OSTEOGLYCIN-ENCODING GENE

被引：36

作者：

UJITA, M

SHINOMURA, T

KIMATA, K

机构：

[1] AICHI MED UNIV,INST MOLEC SCI MED,NAGAKUTE,AICHI 48011,JAPAN

[2] NAGOYA UNIV,SCH AGR SCI,GRAD PROGRAM BIOCHEM REGULAT,NAGOYA,AICHI 46401,JAPAN

来源：

GENE | 1995年 / 158卷 / 02期

关键词：

CARTILAGE; LEUCINE-RICH REPEAT; GLYCOPROTEIN; PROTEOGLYCAN; PROTEOGLYCAN-LB;

D O I：

10.1016/0378-1119(95)00070-M

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Osteoglycin (OG) is a glycoprotein that was first isolated from bovine bone. The deduced amino acid (aa) sequence from the cDNA analysis showed that a precursor of OG has consensus leucine-rich repeats. In this study, we have isolated from a mouse limb-bud library cDNA clones encoding a 298-aa OG. This molecule shows 85 and 86% homology to human and bovine OG, respectively. Furthermore, the C-terminal two-thirds of the protein shows 48% homology to the corresponding portion of chick proteoglycan (PG)-Lb, a PG that has been shown to be preferentially expressed in the zone of flattened chondrocytes in the developing limb cartilage. Northern blot analysis of various mouse tissues revealed a 3.7-kb transcript in a limited number of these tissues.

引用

页码：237 / 240

页数：4

共 18 条

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