STUDIES ON STRUCTURE OF CARBOHYDRATE MOIETY OF RABBIT GAMMAG-GLOBULIN .I.

Information about the structure of rabbit γG-globulin carbohydrate has been obtained with the use of isolated glycosidases and concanavalin A. N-acetyl neuraminic acid and N-acetyl glucosamine were shown to be major terminal sugars. Only a small fraction of the galactose was in a terminal position. Mannose could not be detected in a terminal position and appeared to be internal to both N-acetyl glucosamine and galactose. The following anomeric linkages have been established: N-acetyl glucosamine and galactose have β-linkages while mannose and furose are α-linked. Concanavalin A precipitation studies show that some of the non-terminal mannoses are linked to other sugars through carbon-2. © 1969.