5-METHYLCYTOSINE CONTENT OF NUCLEAR-DNA DURING CHEMICAL HEPATOCARCINOGENESIS AND IN CARCINOMAS WHICH RESULT

被引:175
作者
LAPEYRE, JN [1 ]
BECKER, FF [1 ]
机构
[1] UNIV TEXAS, MD ANDERSON HOSP & TUMOR INST,MED CTR,CTR CANC, EXPTL PATHOL SECT, HOUSTON, TX 77030 USA
关键词
D O I
10.1016/0006-291X(79)92015-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The 5-methylcytosine content of nuclear DNA from nuclear hepatocellular tissues was determined during various phases of hepatic regeneration and carcinogenesis. DNA from premalignant nodules and primary hepatocellular carcinomas induced by exposure to acetylaminofluorene, as well as PHC induced by diethylnitrosamine was undermethylated by 20%, 45%, and 32.5% respectively. Since a 12.5% hypomethylation occurred during the DNA synthetic phase of hepatic regeneration, the effect of cell proliferation on DNA-methylation in malignancies was examined in transplantable hepatocellular carcinomas. The DNA from two transplantable hepatocellular carcinoma lines was less methylated than predicted rates of cell division in these tumors. This finding suggested that an aberration in endogenous DNA methylation may occur during neoplastic transformation. © 1979.
引用
收藏
页码:698 / 705
页数:8
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