CHEMICAL SENSORS BASED ON CONTROLLED-RELEASE POLYMER SYSTEMS

被引：51

作者：

BARNARD, SM ^{[1
]}

WALT, DR ^{[1
]}

机构：

[1] TUFTS UNIV,DEPT CHEM,MAX TISHLER LAB ORGAN CHEM,MEDFORD,MA 02155

来源：

SCIENCE | 1991年 / 251卷 / 4996期

关键词：

D O I：

10.1126/science.2000492

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A novel chemical sensor has been developed in which the polymer ethylene-vinyl acetate is used as a controlled-release system to deliver reagents to the sensing region of an optical fiber for a homogeneous competitive immunoassay based on fluorescence energy transfer. A competition reaction is used to enable continuous measurements of the solution antigen concentration. More generally, the technique allows irreversible indicating chemistries to be used in the construction of chemical sensors that can measure continuously for long periods. Although the sensor configuration has not been optimized in all respects, data are presented for a model system in which a fluorescein-labeled antibody and Texas Red-labeled immunoglobulin G (IgG) are used.

引用

页码：927 / 929

页数：3

共 16 条

[1]

ANDERSON FP, 1988, CLIN CHEM, V34, P1417

[2]

BAWA R, 1985, J CONTROL RELEASE, V1, P259

[3] REGENERABLE FIBER-OPTIC-BASED IMMUNOSENSOR [J].

BRIGHT, FV ;

BETTS, TA ;

LITWILER, KS .

ANALYTICAL CHEMISTRY, 1990, 62 (10) :1065-1069

[4] AN OPTICAL IONIC-STRENGTH SENSOR BASED ON POLY-ELECTROLYTE ASSOCIATION AND FLUORESCENCE ENERGY-TRANSFER [J].

CHRISTIAN, LM ;

SEITZ, WR .

TALANTA, 1988, 35 (02) :119-122

[5]

Forster T, 1959, DISCUSS FARADAY SOC, V27, P7, DOI DOI 10.1039/DF9592700007

[6] CONTROLLED RELEASE OF BIOLOGICALLY-ACTIVE COMPOUNDS FROM BIOERODIBLE POLYMERS [J].

HELLER, J .

BIOMATERIALS, 1980, 1 (01) :51-57

[7] PRESSURIZED MEMBRANE INDICATOR SYSTEM FOR FLUOROGENIC-BASED FIBER-OPTIC CHEMICAL SENSORS [J].

INMAN, SM ;

STROMVALL, EJ ;

LIEBERMAN, SH .

ANALYTICA CHIMICA ACTA, 1989, 217 (02) :249-262

[8]

JANATA J, 1990, ANAL CHEM, V62, pR33, DOI 10.1021/ac00211a017

[9] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY [J].

KOHLER, G ;

MILSTEIN, C .

NATURE, 1975, 256 (5517) :495-497

[10] FIBER-OPTIC SENSORS BASED ON REAGENT DELIVERY WITH CONTROLLED-RELEASE POLYMERS [J].

LUO, SF ;

WALT, DR .

ANALYTICAL CHEMISTRY, 1989, 61 (02) :174-177

← 1 2 →