INHIBITION OF THE RAT RENAL NA+/H+ EXCHANGER BY BETA-ADRENERGIC ANTAGONISTS

被引：3

作者：

FRIEDRICH, T ^{[1
]}

BURCKHARDT, G ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS,W-6000 FRANKFURT 70,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)81236-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The β-adrenergic antagonists, alprenolol and propranolol, inhibit the Na+/H+ exchanger in rat renal brush-border membrane vesicles. Half-maximal inhibition occurs at 86 μM alprenolol and 36 μM propranolol. Similar to amiloride and Na+, propranolol protects the Na+/H+ exchanger from irreversible inhibition by the carboxyl group reagent, N,N′-dicyclohexylcarbodiimide (DCCD). Protection is incomplete, depends on propranolol concentration, and reaches a maximum at 0.4 mM propranolol. With a comparable dose dependence, propranol protects a 65 kDa band from labeling with [14c]DCCD. The data indicate that β-adrenergic antagonists specifically interact with the proximal tubular Na+/H+ exchanger. © 1991 Academic Press, Inc.

引用

页码：311 / 317

页数：7

共 19 条

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