TRANSFORMATION OF CAULIFLOWER (BRASSICA-OLERACEA L VAR BOTRYTIS) - AN EXPERIMENTAL SURVEY

被引：22

作者：

EIMERT, K ^{[1
]}

SIEGEMUND, F ^{[1
]}

机构：

[1] MARTIN LUTHER UNIV,DEPT GENET,DOMPLATZ 1,O-4020 HALLE,GERMANY

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 19卷 / 03期

关键词：

AGROBACTERIUM-TUMEFACIENS; BRASSICA-OLERACEA; ELECTROPORATION; GENETIC TRANSFORMATION; NPTII;

D O I：

10.1007/BF00023396

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The paper compares different approaches for the genetic transformation of cauliflower (Agrobacterium-mediated, PEG-mediated and/or electroporation). Transient expression of the neomycin phosphotransferase II (NPTII) gene could be detected after direct gene transfer. Stable transformation was achieved using both Agrobacterium-mediated and direct gene transfer. Expression as well as incorporation of the NPTII sequence could be demonstrated.

引用

页码：485 / 490

页数：6

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