CLONING OF THE RAT INSULIN-LIKE GROWTH-FACTOR BINDING PROTEIN-1 GENE AND ANALYSIS OF ITS 5' PROMOTER REGION

被引：30

作者：

UNTERMAN, TG

LACSON, RG

MCGARY, E

WHALEN, C

PURPLE, C

GOSWAMI, RG

机构：

[1] VET ADM W SIDE MED CTR,CHICAGO,IL 60680

[2] UNIV ILLINOIS,COLL MED,DEPT MED RES,CHICAGO,IL 60680

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 185卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91725-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To understand specific mechanisms involved in the regulation of insulin-like growth factor binding protein-1 (IGFBP-1), an important modulator of IGF bioactivity, we cloned the rat IGFBP-1 gene and sequenced a 1.5 kb Sph1-Sph1 fragment containing 1110 bases upstream from the translation start site. computer analysis reveals the presence of ATA, CACCC, and CCAAT elements, and putative homeodomain, AP-1, insulin and glucocorticoid response elements in the 5′ promoter. Primer extension and ribonuclease protection studies reveal a single cap site in RNA from rat hepatoma cells and both control and diabetic rat liver. © 1992.

引用

页码：993 / 999

页数：7

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