CA-2+-DEPENDENT TRANSLOCATION OF CYTOSOLIC PROTEINS TO ISOLATED GRANULE SUBPOPULATIONS AND PLASMA-MEMBRANE FROM HUMAN NEUTROPHILS

被引：31

作者：

BORREGAARD, N

KJELDSEN, L

LOLLIKE, K

SENGELOV, H

机构：

[1] Granulocyte Research Laboratory, Department of Hematology L-4042. University Hospital, DK-2100 Copenhagen Ø

来源：

FEBS LETTERS | 1992年 / 304卷 / 2-3期

关键词：

ANNEXIN; EXOCYTOSIS; AZUROPHIL GRANULE; SPECIFIC GRANULE; SECRETORY VESICLE;

D O I：

10.1016/0014-5793(92)80617-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to identify cytosolic proteins involved in control of granule exocytosis in human neutrophils, subcellualr fractions enriched in each of the 3 major granule subsets were incubated with cytosol from neutrophils in the presence or absence of Ca2+. After washing, proteins were eluted from the organelles by EGTA. Annexins I, II, IV and VI were found to bind to all organelles studied. In addition, a 28-kDa protein was found to bind exclusively to plasma membranes and secretory vesicles, the most readily exocytosed organelle of neutrophils. Ca2+-dependent association of cytosolic proteins to different granule subsets may control differential exocytosis of granules.

引用

页码：195 / 197

页数：3

共 9 条

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