PURIFICATION OF CHLAMYDIA-TRACHOMATIS STRAINS IN MIXED INFECTION BY MONOCLONAL-ANTIBODY NEUTRALIZATION

被引：2

作者：

LAMPE, MF

STAMM, WE

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 02期

关键词：

D O I：

10.1128/JCM.32.2.533-535.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A Chlamydia trachomatis D* serovariant strain was found to be mixed with an F serovar strain in a clinical specimen. By using a monoclonal antibody which neutralizes serovar F infectivity in hamster kidney cells, the D* variant strain was enriched until it could be cloned by limiting dilution. This newly described neutralization enrichment procedure can be used to purify a C. trachomatis serovar present in small numbers in a mixed culture or, potentially, to identify nonneutralizable mutants.

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页码：533 / 535

页数：3

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