CLONING AND SEQUENCE-ANALYSIS OF THE RAT AUGMENTOR OF LIVER-REGENERATION (ALR) GENE - EXPRESSION OF BIOLOGICALLY-ACTIVE RECOMBINANT ALR AND DEMONSTRATION OF TISSUE DISTRIBUTION

被引:163
作者
HAGIYA, M
FRANCAVILLA, A
POLIMENO, L
IHARA, I
SAKAI, H
SEKI, T
SHIMONISHI, M
PORTER, KA
STARZL, TE
机构
[1] TOYOBO CO LTD, PHARMACEUT RES CTR, OTSU, SHIGA, JAPAN
[2] UNIV BARI, DEPT GASTROENTEROL, I-70124 BARI, ITALY
[3] UNIV PITTSBURGH, MED CTR, PITTSBURGH TRANSPLANT INST, PITTSBURGH, PA 15213 USA
[4] ST MARYS HOSP & MED SCH, DEPT EXPTL PATHOL, LONDON W2, ENGLAND
关键词
D O I
10.1073/pnas.91.17.8142
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A full-length cDNA clone encoding a purified augmenter of liver regeneration (ALR) factor prepared from the cytosol of weanling rat livers was isolated. The 1.2-kb cDNA included a 299-bp 5' untranslated region, a 375-bp coding region, and a 550-bp 3' untranslated region. It encoded a protein consisting of 125 amino acids. The molecular weight of ALR calculated from the cDNA was 15,081, which is consistent with the size estimated by SDS/PAGE under reducing conditions. The molecular weight of the purified native ALR estimated by SDS/PAGE under nonreducing conditions was approximate to 30,000; thus ALR apparently has a homodimeric structure. The recombinant ALR produced by expression of the cDNA in COS cells was tested in vivo in the canine Eck fistula model and found to have potency equivalent to the purified native ALR. The 125-aa sequence deduced from the rat ALR cDNA shows 50% homology to the amino acid sequence of the gene for oxidative phosphorylation and vegetative growth in the yeast Saccharomyces cerevisiae.
引用
收藏
页码:8142 / 8146
页数:5
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