BRUGIA-PAHANGI - THE EFFECTS OF CECROPINS ON MICROFILARIAE IN-VITRO AND IN AEDES-AEGYPTI

被引：50

作者：

CHALK, R ^{[1
]}

TOWNSON, H ^{[1
]}

HAM, PJ ^{[1
]}

机构：

[1] UNIV LIVERPOOL,LIVERPOOL SCH TROP MED,DIV BIOMED SCI,LIVERPOOL L3 5QA,MERSEYSIDE,ENGLAND

来源：

EXPERIMENTAL PARASITOLOGY | 1995年 / 80卷 / 03期

基金：

英国惠康基金;

关键词：

AEDES AEGYPTI; INSECT ANTIBACTERIAL PEPTIDE; BRUGIA PAHANGI; CECROPIN; NEMATODE; MOSQUITO; IN VITRO TOXICITY; IN VIVO TOXICITY; PBS; PHOSPHATE-BUFFERED SALINE; TC199; TISSUE CULTURE MEDIUM 199;

D O I：

10.1006/expr.1995.1052

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Synthetic cecropins, antibacterial peptides from insect haemolymph, have been tested for their ability to attenuate the motility of microfilariae of the filarial nematode Brugia pahangi in an in vitro assay. Fifty micromolar concentrations of these peptides, equivalent to physiological concentrations in immune-stimulated insects, cause significant attenuation of motility compared with untreated microfilariae. Similar results were obtained with cecropins A and B. This is the lowest concentration for which cecropin has been reported to be active against eukaryote organisms. Antiserum to the cecropin homologue sarcotoxin IA successfully blocked the observed activity. When the same concentration of cecropin B was coinjected with B. pahangi microfilariae into adult females of the mosquito, Aedes aegypti, a significant reduction in the numbers of developing larvae was observed. (C) 1995 Academic Press, Inc.

引用

页码：401 / 406

页数：6

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