COMPARISON OF THE HIV-1 AND HIV-2 PROTEINASES USING OLIGOPEPTIDE SUBSTRATES REPRESENTING CLEAVAGE SITES IN GAG AND GAG-POL POLYPROTEINS

被引：143

作者：

TOZSER, J ^{[1
]}

BLAHA, I ^{[1
]}

COPELAND, TD ^{[1
]}

WONDRAK, EM ^{[1
]}

OROSZLAN, S ^{[1
]}

机构：

[1] NCI, FREDERICK CANC RES & DEV CTR, ABL BASIC RES PROGRAM, MOLEC VIROL & CARCINOGENESIS LAB,POB B, FREDERICK, MD 21702 USA

来源：

FEBS LETTERS | 1991年 / 281卷 / 1-2期

关键词：

HIV-1; PROTEINASE; HIV-2; OLIGOPEPTIDE SUBSTRATE; ENZYME KINETICS; SUBSTRATE SPECIFICITY;

D O I：

10.1016/0014-5793(91)80362-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The substrate specificity of the human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) proteinases was compared using oligopeptides corresponding to cleavage sites in the Gag and Gag-Pol polyproteins of both viruses. All peptides mimicking cleavage sites at the junction of major functional protein domains were correctly cleaved by both enzymes. However, some other peptides thought to represent secondary cleavage sites remained intact. The kinetic parameters (K(m) and k(cat)) obtained for the different substrates showed several hundred-fold variation but were similar for the same substrate.

引用

页码：77 / 80

页数：4

共 29 条

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