ISLET AMYLOID POLYPEPTIDE (IAPP) IS SYNTHESIZED IN THE ISLETS OF LANGERHANS - DETECTION OF IAPP POLYPEPTIDE AND IAPP MESSENGER-RNA BY COMBINED INSITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY IN RAT PANCREAS

被引：9

作者：

DENIJN, M

DEWEGER, RA

VANMANSFELD, ADM

VANUNNIK, JAM

LIPS, CJM

机构：

[1] UNIV HOSP UTRECHT,DEPT PATHOL,DIV HISTOCHEM CYTOCHEM & ELECTRON MICROSCOPY,POB 85500,3508 GA UTRECHT,NETHERLANDS

[2] UNIV UTRECHT,INST MOLEC BIOL & MED BIOL,3584 CH UTRECHT,NETHERLANDS

[3] UNIV HOSP UTRECHT,DIV INTERNAL MED & DERMATOL,3508 GA UTRECHT,NETHERLANDS

[4] UNIV UTRECHT,PHYSIOL CHEM LAB,3584 CH UTRECHT,NETHERLANDS

来源：

HISTOCHEMISTRY | 1992年 / 97卷 / 01期

关键词：

D O I：

10.1007/BF00271279

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We investigated the localization of IAPP mRNA by means of in situ hybridization in tissue sections of rat pancreas. A S-35-labeled, IAPP-specific DNA probe - hybridized specifically in the islets of Langerhans. This localization was confirmed by immunohistochemical localization of insulin and IAPP polypeptides on adjacent tissue sections. Moreover, combined in situ hybridization of IAPP mRNA and immunohistochemistry of insulin and IAPP polypeptide on the same section, using insulin as specific marker shows the presence of IAPP mRNA in the islets of Langerhans.

引用

页码：33 / 37

页数：5

共 18 条

[1]

BELL GI, 1980, NATURE, V284, P26, DOI 10.1038/284026a0

[2] SIMULTANEOUS INSITU DETECTION OF VIRAL-RNA AND ANTIGENS [J].

BRAHIC, M ;

HAASE, AT ;

CASH, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (17) :5445-5448

[3]

CLARK A, 1989, CELL TISSUE RES, V257, P179

[4] AMYLIN FOUND IN AMYLOID DEPOSITS IN HUMAN TYPE-2 DIABETES-MELLITUS MAY BE A HORMONE THAT REGULATES GLYCOGEN-METABOLISM IN SKELETAL-MUSCLE [J].

COOPER, GJS ;

LEIGHTON, B ;

DIMITRIADIS, GD ;

PARRYBILLINGS, M ;

KOWALCHUK, JM ;

HOWLAND, K ;

ROTHBARD, JB ;

WILLIS, AC ;

REID, KBM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7763-7766

[5] PURIFICATION AND CHARACTERIZATION OF A PEPTIDE FROM AMYLOID-RICH PANCREASES OF TYPE-2 DIABETIC-PATIENTS [J].

COOPER, GJS ;

WILLIS, AC ;

CLARK, A ;

TURNER, RC ;

SIM, RB ;

REID, KBM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8628-8632

[6] DETECTION OF CALCITONIN-ENCODING MESSENGER-RNA BY RADIOACTIVE AND NONRADIOACTIVE INSITU HYBRIDIZATION IMPROVED COLORIMETRIC DETECTION AND CELLULAR-LOCALIZATION OF MESSENGER-RNA IN THYROID SECTIONS [J].

DENIJN, M ;

DEWEGER, RA ;

BERENDS, MJH ;

COMPIERSPIES, PI ;

JANSZ, H ;

VANUNNIK, JAM ;

LIPS, CJM .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1990, 38 (03) :351-358

[7] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[8] EXPRESSION OF THE RAT AMYLIN (IAPP DAP) GENE [J].

FERRIER, GJM ;

PIERSON, AM ;

JONES, PM ;

BLOOM, SR ;

GIRGIS, SI ;

LEGON, S .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 1989, 3 (01) :R1-R4

[9] ACETYLATION OF CHROMOSOME SQUASHES OF DROSOPHILA-MELANOGASTER DECREASES BACKGROUND IN AUTORADIOGRAPHS FROM HYBRIDIZATION WITH [I125] LABELED RNA [J].

HAYASHI, S ;

GILLAM, IC ;

DELANEY, AD ;

TENER, GM .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1978, 26 (08) :677-679

[10] INSITU HYBRIDIZATION METHODS FOR THE DETECTION OF SOMATOSTATIN MESSENGER-RNA IN TISSUE-SECTIONS USING ANTISENSE RNA PROBES [J].

HOEFLER, H ;

CHILDERS, H ;

MONTMINY, MR ;

LECHAN, RM ;

GOODMAN, RH ;

WOLFE, HJ .

HISTOCHEMICAL JOURNAL, 1986, 18 (11-12) :597-&

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