EFFECTS OF DIMETHYL-SULFOXIDE ON HEAVY-CHAIN MONOCLONAL-ANTIBODY PRODUCTION FROM PLANT-CELL CULTURE

被引：26

作者：

WAHL, MF ^{[1
]}

AN, GH ^{[1
]}

LEE, JM ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,INST BIOL CHEM,PULLMAN,WA 99164

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 05期

关键词：

D O I：

10.1007/BF00132011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The addition of 4% (v/v) dimethyl sulfoxide (DMSO) increased the total production of heavy chain monoclonal antibody (HC MAb) in suspension culture of genetically modified Nicotiana tabacum by three fold. This may be due to stabilization of HC MAb in the presence of DMSO by forming hydrogen bonds with proton-donor groups on protein molecules.

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页码：463 / 468

页数：6

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