WAYS OF COLLAGEN SEPARATION IN PATHOLOGICALLY ALTERED TISSUE

被引：13

作者：

ADAM, M ^{[1
]}

DEYL, Z ^{[1
]}

MACEK, K ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI, INST PHYSIOL, PRAGUE, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 162卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)81909-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A system of chromatographic methods using two successive DEAE-cellulose chromatographic steps and two successive separations on Bio-Gel A-1.5 m has been worked out for the separation of individual collagen types. The success of the procedure is based on the preliminary removal of proteoglycans during the first DEAE-cellulose run. Alternatively it is possible to replace chromatographic steps, following the removal of proteoglycans, with fraction precipitation. © 1979.

引用

页码：163 / 168

页数：6

共 8 条

[1]

ADAM M, 1976, CLIN CHIM ACTA, V69, P53, DOI 10.1016/0009-8981(76)90470-8

[2] COLLAGEN IN RHEUMATOID-ARTHRITIS [J].

ADAM, M ;

VITASEK, R ;

DEYL, Z ;

FELSCH, G ;

MUSILOVA, J ;

OLSOVSKA, Z .

CLINICA CHIMICA ACTA, 1976, 70 (01) :61-69

[3] PREPARATION OF TYPE-III PROCOLLAGEN AND COLLAGEN FROM RAT SKIN [J].

BYERS, PH ;

MCKENNEY, KH ;

LICHTENSTEIN, JR ;

MARTIN, GR .

BIOCHEMISTRY, 1974, 13 (25) :5243-5248

[4] COLLAGEN POLYMORPHISM - CHARACTERIZATION OF MOLECULES WITH CHAIN COMPOSITION [ALPHA-1(III)]3 IN HUMAN TISSUES [J].

CHUNG, E ;

MILLER, EJ .

SCIENCE, 1974, 183 (4130) :1200-1201

[5] ISOLATION AND CHARACTERIZATION OF CYANOGEN-BROMIDE PEPTIDES FROM ALPHA 1 (III) CHAIN OF HUMAN COLLAGEN [J].

CHUNG, E ;

KEELE, EM ;

MILLER, EJ .

BIOCHEMISTRY, 1974, 13 (17) :3459-3464

[6] ISOLATION AND CHARACTERIZATION OF A COLLAGEN FROM CHICK CARTILAGE CONTAINING 3 IDENTICAL ALPHA-CHAINS [J].

MILLER, EJ .

BIOCHEMISTRY, 1971, 10 (09) :1652-&

[7] DISTURBANCE IN REGULATION OF TYPE OF COLLAGEN SYNTHESIZED IN A FORM OF OSTEOGENESIS IMPERFECTA [J].

MULLER, PK ;

LEMMEN, C ;

GAY, S ;

MEIGEL, WN .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 59 (01) :97-104

[8]

Ramachandran G. N., 1976, BIOCH COLLAGEN

← 1 →