SINGLET OXYGEN INDUCED SINGLE-STRAND BREAKS IN PLASMID-PBR322 DNA - THE ENHANCING EFFECT OF THIOLS

被引：58

作者：

DEVASAGAYAM, TPA ^{[1
]}

DI MASCIO, P ^{[1
]}

KAISER, S ^{[1
]}

SIES, H ^{[1
]}

机构：

[1] UNIV DUSSELDORF, INST PHYSIOL CHEM 1, MOORENSTR 5, W-4000 DUSSELDORF 1, GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1088卷 / 03期

关键词：

SINGLET OXYGEN; SINGLE-STRAND BREAK; DNA DAMAGE; THIOL;

D O I：

10.1016/0167-4781(91)90133-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biologically occurring thiols, glutathione, cysteamine and cysteine, significantly enhance the single-strand breaks in plasmid pBR322 DNA induced by singlet molecular oxygen (O-1(2)) generated by the thermodissociation of the endoperoxide of 3,3'-(1,4-naphthylidene)dipropionate. The enhancing effect was also observed with chemically related sulfhydryl compounds but not by disulfides. In contrast, dihydrolipoate and its disulfide lipoate protected the plasmid DNA. Metal chelators as well as superoxide dismutase or catalase had no effect, whereas mannitol or sodium azide, decreased the thiol-O-1(2)-induced strand breaks. It is concluded that the observed effects are mediated by reactive oxidation products arising from the O-1(2)-oxidation of thiols.

引用

页码：409 / 412

页数：4

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