A PUTATIVE RNA VIRUS IN BABESIA-BOVIS

被引：21

作者：

JOHNSTON, RC

FARIAS, NAR

GONZALES, JC

DEWES, H

MASUDA, A

TERMIGNONI, C

AMAKO, K

OZAKI, LS

机构：

[1] UNIV FED RIO GRANDE SUL,DEPT BIOTECHNOL,POB 15005,BR-91500 PORTO ALEGRE,RS,BRAZIL

[2] UNIV FED RIO GRANDE SUL,SCH VET,BR-90000 PORTO ALEGRE,RS,BRAZIL

[3] KYUSHU UNIV,SCH MED,DEPT BACTERIOL,FUKUOKA 812,JAPAN

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1991年 / 45卷 / 01期

关键词：

BABESIA-BOVIS; BABESIA-BIGEMINA; PROTOZOAN VIRUS; VIRUS-LIKE PARTICLE;

D O I：

10.1016/0166-6851(91)90037-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Babesia bovis is an intraerythrocytic protozoan that causes bovine babesiosis. Agarose gel electrophoresis of nucleic acids extracted from two isolates of B. bovis reveals, besides bulk DNA, an ethidium bromide-stainable band at about 5.5 kb. Further characterization of the latter with DNase I, RNase and mung bean nuclease suggested it to be a double-stranded RNA. Sonicated parasites were fractionated in a CsCl buoyant density gradient. A sample containing the 5.5-kb RNA was analysed under an electron microscope and a virus-like particle was observed.

引用

页码：155 / 158

页数：4

共 13 条

[1] USE OF CF 12 COLUMNS FOR PREPARATIONS OF DNA FROM RODENT MALARIAS [J].