EFFECT OF CONCURRENT GRAFT-VERSUS-HOST REACTION ON TISSUE DISTRIBUTION AND INFECTIOUS TITER OF MURINE CYTOMEGALOVIRUS

被引:4
作者
CRAY, C
CROUSE, C
ATHERTON, SS
LEVY, RB
机构
[1] UNIV MIAMI,SCH MED,DEPT MICROBIOL & IMMUNOL,MIAMI,FL 33101
[2] UNIV MIAMI,SCH MED,DEPT OPHTHALMOL,MIAMI,FL 33101
关键词
D O I
10.1007/BF01316747
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Infection with cytomegalovirus is a major concern following bone marrow transplantation. Previously, we have described a model whereby infection by MCMV concurrent with the injection of parental strain lymphoid cells into F1 recipient mice results in a severe graft-versus-host reaction (GvHR) when the donor and host differ at the class I MHC locus (i.e., class I MHC disparate GvHR). The present studies were performed to determine whether one consequence of this severe GvHR is the alteration of the tissue distribution and/or levels of infectious virus as compared to mice not undergoing GvHR. Using PCR to detect MCMV DNA, it was observed that the tissue distribution of virus 3 days after injection was identical in recipients of virus alone and of GvHR plus virus inocula. At two weeks post injection, virus was recovered from the salivary gland and pancreas in both groups. In contrast to recipients of MCMV alone, lung tissue from GvHR plus virus mice also contained viral DNA. Notably, these results were corroborated by the plaque assay. Moreover, salivary gland and pancreas from recipients of GvHR plus virus were found to contain higher titers of infectious virus. In total, the data demonstrate that, with the notable exception of the lung, the distribution of virus was not changed in the animals despite the presence of a concurrent severe GvHR. However, viral replication in infected tissues appeared less restricted in these recipients.
引用
收藏
页码:101 / 110
页数:10
相关论文
共 21 条
[1]   GENETIC-CONTROL OF MURINE CYTOMEGALOVIRUS-INFECTION - VIRUS TITERS IN RESISTANT AND SUSCEPTIBLE STRAINS OF MICE [J].
ALLAN, JE ;
SHELLAM, GR .
ARCHIVES OF VIROLOGY, 1984, 81 (1-2) :139-150
[2]   PRETRANSPLANT HERPESVIRUS SEROLOGY AND ACUTE GRAFT-VERSUS-HOST DISEASE [J].
BOSTROM, L ;
RINGDEN, O ;
SUNDBERG, B ;
LINDE, A ;
TOLLEMAR, J ;
NILSSON, B .
TRANSPLANTATION, 1988, 46 (04) :548-552
[3]   RESISTANCE TO MURINE CYTOMEGALOVIRUS LINKED TO MAJOR HISTOCOMPATIBILITY COMPLEX OF MOUSE [J].
CHALMER, JE ;
MACKENZIE, JS ;
STANLEY, NF .
JOURNAL OF GENERAL VIROLOGY, 1977, 37 (OCT) :107-114
[4]   THE PRESENCE OF INFECTIOUS VIRUS BUT NOT CONVENTIONAL ANTIGEN CAN EXACERBATE GRAFT-VERSUS-HOST REACTIONS [J].
CRAY, C ;
LEVY, RB .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1990, 32 (02) :177-182
[5]   THE ABILITY OF MURINE CYTOMEGALOVIRUS AND CLASS-I MAJOR HISTOCOMPATABILITY COMPLEX DISPARATE PARENTAL CELLS TO INDUCE ALTERATIONS CHARACTERISTIC OF SEVERE GRAFT-VERSUS-HOST REACTIONS [J].
CRAY, C ;
LEVY, RB .
TRANSPLANTATION, 1989, 48 (06) :1057-1063
[6]   VIRUS-ASSOCIATED IMMUNE-RESPONSES IN MICE UNDERGOING GVHR EXACERBATED BY CONCURRENT MCMV INFECTION [J].
CRAY, C ;
LEVY, RB .
TRANSPLANTATION, 1990, 50 (06) :1027-1032
[7]   MOLECULAR-CLONING AND SEQUENCING OF THE MTV-1 LTR - EVIDENCE FOR A LTR SEQUENCE ALTERATION [J].
CROUSE, CA ;
PAULEY, RJ .
VIRUS RESEARCH, 1989, 12 (02) :123-138
[8]  
GRATAMA JW, 1987, LANCET, V1, P471
[9]   AUGMENTATION OF GRAFT-VERSUS-HOST REACTION BY CYTOMEGALO-VIRUS INFECTION RESULTING IN INTERSTITIAL PNEUMONITIS [J].
GRUNDY, JE ;
SHANLEY, JD ;
SHEARER, GM .
TRANSPLANTATION, 1985, 39 (05) :548-553
[10]  
GRUNDY JE, 1987, LANCET, V2, P996