DETERMINATION OF VINORELBINE (NAVELBINE) IN TUMOR-CELLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：22

作者：

DEBAL, V

MORJANI, H

MILLOT, JM

ANGIBOUST, JF

GOURDIER, B

MANFAIT, M

机构：

[1] UFR PHARM,SPECT BIOMOLEC LAB,51 RUE COGNACQ JAY,F-51096 REIMS,FRANCE

[2] CHR REIMS,HOP ROBERT DEBRE,PHARM CLIN LAB,F-51092 REIMS,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 581卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80451-U

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method has been developed for the determination within tumour cells of a new vinca alkaloid, vinorelbine. Extractions of vinorelbine from cells were carried out using absolute ethanol. The extracts were injected into a reversed-phase system consisting of two Novapak C18 columns connected in series. The mobile phase was acetonitrile-phosphate buffer, pH 2.7 (60:40, v/v). Using a fluorescence detection, the limit of determination was 8 pmol injected. This method would be suitable for studying the cellular pharmacokinetics of vinorelbine in patients.

引用

页码：93 / 99

页数：7

共 15 条

[1] PHARMACOKINETICS OF A NEW ANTICANCER DRUG, NAVELBINE, IN PATIENTS - COMPARATIVE-STUDY OF RADIOIMMUNOLOGIC AND RADIOACTIVE DETERMINATION METHODS [J].