FLUORESCENCE INSITU HYBRIDIZATION TO DETERMINE ENGRAFTMENT STATUS AFTER MURINE BONE-MARROW TRANSPLANT

被引：22

作者：

HAWKINS, AL ^{[1
]}

JONES, RJ ^{[1
]}

ZEHNBAUER, BA ^{[1
]}

ZICHA, MS ^{[1
]}

COLLECTOR, MJ ^{[1
]}

SHARKIS, SJ ^{[1
]}

GRIFFIN, CA ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV HOSP,CTR ONCOL,1-109,600 N WOLFE ST,BALTIMORE,MD 21205

来源：

CANCER GENETICS AND CYTOGENETICS | 1992年 / 64卷 / 02期

关键词：

D O I：

10.1016/0165-4608(92)90345-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.

引用

页码：145 / 148

页数：4

共 16 条

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