SUPPRESSION OF PEROXISOMAL LIPID BETA-OXIDATION ENZYMES BY TNF-ALPHA

被引：42

作者：

BEIER, K

VOLKL, A

FAHIMI, HD

机构：

[1] Institut für Anatomie und Zellbiologie II, Universiteit Heidelberg, Heidelberg

来源：

FEBS LETTERS | 1992年 / 310卷 / 03期

关键词：

PEROXISOME; TUMOR-NECROSIS-FACTOR; IMMUNOCYTOCHEMISTRY; LIPID BETA-OXIDATION; HYPERLIPIDEMIA;

D O I：

10.1016/0014-5793(92)81347-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

TNF-alpha is a potent cytokine which induces marked hyperlipidemia. Because of the important role of peroxisomes in lipid metabolism we investigated the effects of human recombinant TNF-alpha upon rat liver peroxisomal enzymes. Sixteen hours after the administration of a single dose of 25 mug of TNF-alpha to male rats the activity of peroxisomal fatty acyl-CoA oxidase was reduced by 50%. This was confirmed also by immunoblotting and by quantitative immunoelectron microscopy which in addition revealed substantial reduction of the trifunctional protein (hydratase-dehydrogenase-isomerase) in peroxisomes. These observations suggest that the suppression of peroxisomal,8-oxidation may contribute to the perturbation of the lipid metabolism induced by TNF-alpha.

引用

页码：273 / 276

页数：4

共 24 条

[1] APPLICATION OF AUTOMATIC IMAGE-ANALYSIS FOR QUANTITATIVE MORPHOLOGICAL-STUDIES OF PEROXISOMES IN RAT-LIVER IN CONJUNCTION WITH CYTOCHEMICAL STAINING WITH 3-3'-DIAMINOBENZIDINE AND IMMUNOCYTOCHEMISTRY [J].