NOTES ON A LIGHT AND ELECTRON-MICROSCOPIC DOUBLE-LABELING METHOD COMBINING ANTEROGRADE TRACING WITH PHASEOLUS-VULGARIS LEUKOAGGLUTININ AND RETROGRADE TRACING WITH CHOLERA-TOXIN SUBUNIT-B
被引:52
作者:
BRUCE, K
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机构:
MICHIGAN STATE UNIV, DEPT ANAT, B505 W FEE HALL, E LANSING, MI 48824 USAMICHIGAN STATE UNIV, DEPT ANAT, B505 W FEE HALL, E LANSING, MI 48824 USA
BRUCE, K
[1
]
GROFOVA, I
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h-index: 0
机构:
MICHIGAN STATE UNIV, DEPT ANAT, B505 W FEE HALL, E LANSING, MI 48824 USAMICHIGAN STATE UNIV, DEPT ANAT, B505 W FEE HALL, E LANSING, MI 48824 USA
GROFOVA, I
[1
]
机构:
[1] MICHIGAN STATE UNIV, DEPT ANAT, B505 W FEE HALL, E LANSING, MI 48824 USA
PHASEOLUS-VULGARIS LEUKOAGGLUTININ;
CHOLERA TOXIN SUBUNIT-B;
DOUBLE IMMUNOHISTOCHEMISTRY;
ELECTRON MICROSCOPY;
D O I:
10.1016/0165-0270(92)90040-K
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
Investigations of monosynaptic connections in the central nervous system have been hindered by the lack of compatible markers that can be used at both light and electron microscopic levels. In attempts to determine synaptic contacts between fibers originating in the substantia nigra and neurons projecting to the spinal cord, we have developed a double immunolabeling technique using anterograde transport of Phaseolus vulgaris leucoagglutinin (PHA-L) and retrograde transport of unconjugated cholera toxin subunit B (CTB). In this report, we describe technical modifications which consistently produced superior labeling together with adequate ultrastructural preservation of the tissue and discuss the advantages of the two tracers.