EARLY DETECTION OF CATTLE GRUB (HYPODERMA-LINEATUM AND H-BOVIS) (DIPTERA, OESTRIDAE) USING ELISA

被引:44
作者
COLWELL, DD [1 ]
BARON, RW [1 ]
机构
[1] AGR CANADA,RES STN,LIVESTOCK SCI SECT,POB 3000 MAIN,LETHBRIDGE T1J 4B1,ALBERTA,CANADA
关键词
cattle grubs; ELISA; H.bovis; Hypoderma lineatum; hypodermosis; warble flies;
D O I
10.1111/j.1365-2915.1990.tb00257.x
中图分类号
Q96 [昆虫学];
学科分类号
摘要
ABSTRACT. .An enzyme‐linked immunosorbent assay (ELISA) to detect cattle grub‐infested animals in the autumn in Alberta has been developed. Antibody to Hypoderma lineatum de Vill. was detectable as early as 6 weeks post‐infestation in artificially infested steers. Peak antibody concentrations preceded the peak in maximum ‘apparent’ grub numbers which occurred between 37 and 43 weeks after infestation. Natural infestations with H. lineatum and H. bovis L., ranging from one to ninety‐two grubs per calf, were readily detected by November and the incidence of false positives in uninfested calves was only 5%. Low level (1–4 grubs) infestations of H. bovis only became detectable in February with peak antibody concentrations occurring at the end of April. Prevalence of cattle grub infestations in southern Alberta was shown to be 37% during this study. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码:35 / 42
页数:8
相关论文
共 32 条
[1]  
Baron R.W., Weintraub J., Immunization of cattle against hypodermatosis (Hypoderma lineatum de Vill. and H.bovis L.) using H.lineatum antigens, Veterinary Parasitology, 21, pp. 43-50, (1986)
[2]  
Barrett C.C., A new technique for collecting cattle grub larvae, Southwestern Entomologist, 6, pp. 144-146, (1981)
[3]  
Boulard C., Evolution des anticorps circulants chez les bovins traites contre l'hypodermose, Annates de Recherches Veterinaires, 6, pp. 143-154, (1975)
[4]  
Boulard C., Avantages de l'immunodiagnostic de ľhypodermose bovine etabli par hemagglutination passive et par ELISA, a partir du serum et du lactoserum, sur la numeration des varons, Annates de Recherches Veterinaires, 16, pp. 335-343, (1985)
[5]  
Boulard C., Weintraub J., Immunological response of rabbits artificially infested with the cattle grubs Hypoderma bovis (L.) and H.lineatum (De Vill.) (Diptera, Oestridae), International Journal of Parasitology, 3, pp. 379-386, (1973)
[6]  
Bradford M., A rapid and sensitive method for quantification of microgram quantities of proteins utilizing the principle of protein‐dye binding, Analytical Biochemistry, 72, pp. 248-254, (1976)
[7]  
Burillon G., Messean A., Comparison of two methods of estimation of the warble fly, Warble Fly Control in Europe, pp. 131-140, (1984)
[8]  
Drummond R.O., Economic aspects of ectoparasites of cattle in North America, The Economic Impact of Parasitism in cattle, pp. 9-24, (1987)
[9]  
Gingrich R.E., Acquired resistance to Hypoderma lineatum, comparative immune response of resistant and susceptible cattle, Veterinary Parasitology, 9, pp. 233-242, (1982)
[10]  
Gregson J.D., Holland G.P., Device for charting and obtaining naturally emerged cattle warbles (Diptera, Oestridae), Entomological Society of British Columbia, Proceedings, 41, pp. 31-33, (1944)