INVESTIGATION OF PHOSPHOLIPID CHANGES IN ACTIVELY METABOLIZING CELLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF P-32-LABELED PHOSPHOLIPIDS

被引：11

作者：

BLOM, CP

DEIERKAUF, FA

RIEMERSMA, JC

机构：

[1] Laboratory for Medical Chemistry, Sylvius Laboratories, University of Leiden, Wassenaarseweg 72

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 171卷 / APR期

关键词：

D O I：

10.1016/S0021-9673(01)95312-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic system was employed to study turnover rates of phospholipids in various types of cells, such as leukocytes, erythrocytes and yeast cells. In the extracts obtained from 32P-labelled cells ten to twelve different phospholipids could be identified. Phosphatidyl glycerol and other minor phospholipids show a high specific activity, so that the lipids may be readily detected. The reproducibility and sensitivity of the method permits the determination of lipid changes during nutrient uptake, phagocytosis, or other cellular activities. © 1979.

引用

页码：331 / 338

页数：8

共 23 条

[1] LIQUID-CHROMATOGRAPHY OF LIPIDS - CRITICAL REVIEW [J].