MICROPROBE TECHNIQUES FOR DETERMINING DIFFUSIVITIES AND RESPIRATION RATES IN MICROBIAL SLIME SYSTEMS

被引:108
作者
BUNGAY, HR
WHALEN, WJ
SANDERS, WM
机构
[1] Department of Environmental Systems, Engineering, Clemson University, Clemson
[2] St. Vincent Charity Hospital, Cleveland, Ohio
[3] Department of the Interior, Athens, Georgia
关键词
D O I
10.1002/bit.260110505
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A microprobe electrode was used to determine dissolved oxygen concentrations near the surface and within a bacterial slime mass supplied with a continuous flow of nutrient solution. With dilute medium, the oxygen profile became level at high concentrations within the film, indicating substrate‐limited respiration. More concentrated medium caused the profile to fall to low oxygen concentrations characteristic of oxygen‐limited respiration. Oxygen responses to sudden changes in concentration of nutrient medium were measured. Estimates of microbial respiration rate and of diffusivity of oxygen were based on well‐known diffusion equations. Copyright © 1969 John Wiley & Sons, Inc.
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页码:765 / &
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