EFFECT OF LIPOPOLYSACCHARIDE FROM PORPHYROMONAS-GINGIVALIS ON PROSTAGLANDIN-E2 AND INTERLEUKIN-1 BETA RELEASE FROM RAT PERIOSTEAL AND HUMAN GINGIVAL FIBROBLASTS INVITRO

被引：63

作者：

SISMEYDURRANT, HJ ^{[1
]}

HOPPS, RM ^{[1
]}

机构：

[1] UNIV LONDON LONDON HOSP,COLL MED,DEPT ORAL PATHOL,LONDON E1 1BB,ENGLAND

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 6卷 / 06期

关键词：

PORPHYROMONAS-GINGIVALIS; LIPOPOLYSACCHARIDE; FIBROBLAST; PROSTAGLANDIN-E2; INTERLEUKIN-1;

D O I：

10.1111/j.1399-302X.1991.tb00510.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Lipopolysaccharide (LPS) was extracted from Porphyromonas gingivalis (W83) by the Westphal procedure, nuclease-digested and ultracentrifuged. Fibroblasts were obtained from human gingival tissue and rat periosteum, grown to confluence then stimulated in serum-free medium with 0.1, 1.0 and 10.0-mu-g/ml LPS. The levels of prostaglandin E2 (PGE2) and interleukin-1-beta (IL-1-beta) released were measured after 2, 4 and 6 d by specific radioimmunoassays. Unstimulated gingival fibroblasts produced low levels of PGE2 (24.5 +/- 1.5 (SD) ng/ml) and IL-1-beta (0.34 +/- 0.29 ng/ml). LPS stimulated statistically significant dose-related increases in PGE2 and IL-1-beta at the concentrations of LPS tested. At 10.0-mu-g/ml, LPS-stimulated fibroblasts produced 363.5 +/- 40.3 ng/ml PGE2 and 1.81 +/- 0.1 ng/ml IL-1-beta in 6 d. These results demonstrate that LPS from P. gingivalis is capable of stimulating PGE2 and IL-1-beta release from fibroblasts. This would appear to be an additional mechanism by which LPS can induce tissue breakdown in periodontal disease.

引用

页码：378 / 380

页数：3

共 20 条

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