MOLECULAR-STRUCTURE OF RAS-RELATED SMALL GTP-BINDING PROTEIN GENES OF RICE PLANTS AND GTPASE ACTIVITIES OF GENE-PRODUCTS IN ESCHERICHIA-COLI

被引：19

作者：

KIDOU, S

ANAI, T

UMEDA, M

AOTSUKA, S

TSUGE, T

KATO, A

UCHIMIYA, H

机构：

[1] UNIV TOKYO,INST MOLEC & CELLULAR BIOSCI,TOKYO 113,JAPAN

[2] HOKKAIDO UNIV,DEPT BOT,SAPPORO,HOKKAIDO 060,JAPAN

[3] POLA CORP,PLANT BIOSCI LAB,TOTSUKA KU,YOKOHAMA 244,JAPAN

[4] NISSHINBO IND INC,TOKYO RES CTR,ADACHI KU,TOKYO 123,JAPAN

来源：

FEBS LETTERS | 1993年 / 332卷 / 03期

关键词：

CDNA; RICE; SMALL GTP-BINDING PROTEIN; GTPASE; YPT RAB;

D O I：

10.1016/0014-5793(93)80650-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated two rice cDNA clones (ric1 and ric2) encoding proteins homologous to the ras-related small GTP-binding protein. The amino acid sequences of ric1 and ric2 are conserved in four regions involved in GTP binding and hydrolysis which are characteristic in the ras and ras-related small GTP-binding protein genes. In addition, two consecutive cysteine residues near the carboxyl-terminal end required for membrane anchoring are also present in ric1 and ric2. The ric1 and ric2 proteins synthesized in Escherichia coli possessed GTPase activity (i.e. hydrolysis of GTP to GDP).

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收藏

页码：282 / 286

页数：5

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