THE GUINEA-PIG HISTAMINE H-2-RECEPTOR - GENE CLONING, TISSUE EXPRESSION AND CHROMOSOMAL LOCALIZATION OF ITS HUMAN COUNTERPART

被引：33

作者：

TRAIFFORT, E ^{[1
]}

VIZUETE, ML ^{[1
]}

TARDIVELLACOMBE, J ^{[1
]}

SOUIL, E ^{[1
]}

SCHWARTZ, JC ^{[1
]}

RUAT, M ^{[1
]}

机构：

[1] CTR PAUL BROCA,UNITE NEUROBIOL & PHARMACOL,INSERM,U109,F-75014 PARIS,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 211卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1851

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The guinea pig is the prototypic animal species for the histamine H-2 receptor. Using a strategy based upon nucleotide sequence homology and starting from the sequence of the rat histamine H-2 receptor (Ruat et al,, Biochem. Biophys. Res. Commun. 1991, 179: 1470-78), we have cloned an intronless highly homologous DNA very likely encoding the guinea pig H-2 receptor. The encoded 359 amino acid protein displays 83 to 86% identity with the rat-, human- or dog-H-2 receptors. Northern blot analysis identified a single transcript of 4.6 kb in peripheral tissues and brain areas in which the presence of the H-2 receptor had been revealed previously by either photoaffinity labeling or binding studies. In brain, the distribution of transcripts, established by either Northern blots or ill situ hybridization studies, was consistent with the localization of the H-2-receptor. In addition, using Southern analysis of a chromosome mapping panel constructed from human x hamster hybridomas, we assigned the H-2 receptor gene to human chromosome 5. (C) Academic Press, Inc.

引用

页码：570 / 577

页数：8

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