GENETIC-CONTROL OF DIHYDROFLAVONOL 4-REDUCTASE GENE-EXPRESSION IN PETUNIA-HYBRIDA

被引：66

作者：

HUITS, HSM ^{[1
]}

GERATS, AGM ^{[1
]}

KREIKE, MM ^{[1
]}

MOL, JNM ^{[1
]}

KOES, RE ^{[1
]}

机构：

[1] VRIJE UNIV AMSTERDAM,INST MOLEC BIOL SCI,BIOCENTRUM AMSTERDAM,DEPT GENET,1081 HV AMSTERDAM,NETHERLANDS

来源：

PLANT JOURNAL | 1994年 / 6卷 / 03期

关键词：

D O I：

10.1046/j.1365-313X.1994.06030295.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The functions of four loci (An1, An2, An4, and An6) which control pigmentation in flowers of Petunia hybrida have been characterized. Linkage-analysis and molecular complementation experiments showed that the An6 locus contains the structural dfrA gene, encoding the enzyme dihydroflavonol 4-reductase (DFR). Analysis of gus gene expression driven by the dfrA promoter in transgenic plants showed that the dfrA promoter is highly active in the flower corolla, the anthers and seeds and, at a lower level, in ovules and the flower stem. These data are discussed in relation to the expression of other pigmentation genes and the accumulation pattern of anthocyanins. The expression of the dfrA-gus transgene was dependent on the genes an1 (in every tissue), an2 (in the flower limb only) and an4 (in anthers), demonstrating that these genes encode regulatory factors that control dfrA promoter activity.

引用

页码：295 / 310

页数：16

共 70 条

[1] GENETIC INTERACTIONS UNDERLYING FLOWER COLOR PATTERNS IN ANTIRRHINUM-MAJUS [J].