GAS-CHROMATOGRAPHIC ELECTRON-IMPACT MASS-SPECTROMETRIC SCREENING-PROCEDURE FOR UNKNOWN HYDROXYALDEHYDIC LIPID-PEROXIDATION PRODUCTS AFTER PENTAFLUOROBENZYLOXIME DERIVATIZATION

被引：22

作者：

LOIDLSTAHLHOFEN, A ^{[1
]}

KERN, W ^{[1
]}

SPITELLER, G ^{[1
]}

机构：

[1] UNIV BAYREUTH,LEHRSTUHL ORGAN CHEM 1,D-95440 BAYREUTH,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 673卷 / 01期

关键词：

D O I：

10.1016/0378-4347(95)00244-D

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aldehydic lipid peroxidation products can be detected after transformation to pentafluorobenzyloxime derivatives by GC-MS screening using characteristic ion traces. Thus the rather unstable unsaturated hydroxyaldehyde, 6-hydroxy-2,4-undecadienal, was identified as autoxidation product of linoleic acid. Its structure was unambiguously confirmed by comparison with an authentic sample. After Fe2+-ascosbate induced lipid peroxidation of oleic acid several 4-hydroxy-2-alkenals and 4-hydroxyalkanals were detected. These represent previously unknown secondary oxidation products of lipid peroxidation of oleic acid. Nevertheless oleic acid proved about 1000 times more stable against peroxidation than linoleic or higher unsaturated acids.

引用

页码：1 / 14

页数：14

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