A DIAGNOSTIC METHOD TO DETECT ALCELAPHINE HERPESVIRUS-1 OF MALIGNANT CATARRHAL FEVER USING THE POLYMERASE CHAIN-REACTION

被引：26

作者：

HSU, D

SHIH, LM

CASTRO, AE

ZEE, YC

机构：

[1] UNIV CALIF DAVIS, DEPT VET MICROBIOL & IMMUNOL, DAVIS, CA 95616 USA

[2] UNIV CALIF DAVIS, CALIF VET DIAGNOST LAB SYST, DAVIS, CA 95616 USA

来源：

ARCHIVES OF VIROLOGY | 1990年 / 114卷 / 3-4期

关键词：

D O I：

10.1007/BF01310755

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A sensitive diagnostic method specific for alcelaphine herpesvirus-1, causative agent of malignant catarrhal fever, has been developed. Based on the nucleotide sequence of the alcelaphine herpesvirus-1 genomic DNA, a pair of 30 nucleotide primers was selected and synthesized for detecting the virus genome using the polymerase chain reaction (PCR). The virus genome was detected in crude cell lysate using the amplification reaction. © 1990 Springer-Verlag.

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页码：259 / 263

页数：5

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