UTILITY OF HUMAN BONE-MARROW OBTAINED INCIDENTAL TO ORTHOPEDIC-SURGERY FOR HEMATOPOIETIC CLONAL ASSAYS

被引：14

作者：

VOLPE, DA

DU, DL

POHL, KP

CAMPBELL, JP

MURPHY, MJ

机构：

[1] HIPPLE CANC RES CTR,4100 S KETTERING BLVD,DAYTON,OH 45439

[2] KETTERING MED CTR,DAYTON,OH

来源：

PATHOBIOLOGY | 1991年 / 59卷 / 01期

关键词：

FEMORAL; BONE MARROW; COLONY-FORMING UNITS ASSAY; HUMAN; CFU-GM; BFU-E; CFU-E; MICROCAPILLARY;

D O I：

10.1159/000163615

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe the establishment of a convenient method of acquiring human bone marrow cells for use in microcapillary clonal assays. Femoral epiphyseal and diaphyseal bone fragments and femoral canal reamings were collected incidental to total hip replacement surgery and cultured for human granulocyte-macrophage colony-forming units, erythroid burst-forming units and erythroid colony-forming units. This readily available source of normal human marrow provides an abundant quantity of hematopoietic progenitors of documented normalcy.

引用

页码：53 / 56

页数：4

共 8 条

[1]

BURGESS AW, 1977, BLOOD, V49, P573

[2]

DELAFOREST RBG, 1979, EXP HEMATOL, V7, P334

[3] MICROCAPILLARY CLONOGENIC ASSAYS FOR HUMAN MARROW HEMATOPOIETIC PROGENITOR CELLS [J].

DU, DL ;

VOLPE, DA ;

MURPHY, MJ .

INTERNATIONAL JOURNAL OF CELL CLONING, 1989, 7 (05) :303-313

[4]

DU DL, 1990, CANCER RES, V50, P4038

[5]

DU DL, IN PRESS EXP HEMATOL

[6]

GALLICCHIO VS, 1979, EXP HEMATOL, V7, P219

[7]

GOLDE DW, 1978, CELLULAR MOL REGULAT, P305

[8]

WITTBERG KL, 1986, EXP CELL BIOL, V53, P41

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