CLONING OF THE HLA CLASS-II REGION IN YEAST ARTIFICIAL CHROMOSOMES

被引：50

作者：

RAGOUSSIS, J ^{[1
]}

MONACO, A ^{[1
]}

MOCKRIDGE, I ^{[1
]}

KENDALL, E ^{[1
]}

CAMPBELL, RD ^{[1
]}

TROWSDALE, J ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,MRC,IMMUNOCHEM UNIT,OXFORD,ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 09期

关键词：

MAJOR HISTOCOMPATIBILITY COMPLEX; HLA-DR MOLECULES; CLASS-III; LARGE-SCALE CLONING; HUMAN GENOME;

D O I：

10.1073/pnas.88.9.3753

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Yeast artificial chromosomes (YACs) have been applied to clone the entire class II region of the human major histocompatibility complex (MHC), including its flanking regions, in a contig over 1.5 million base pairs (bp) long. The human DNA inserts in the YACs have a size between 60 and 1300 kbp and were isolated from two EcoRI partial digest libraries. The gaps between DRA and DRB, DRB and DQA, and DOB and DPA, which had not been cloned by other means, have been bridged with YAC clones. The contig extends through the 400 kbp of DNA between the DRA and C4 genes, thus linking the class II region with the complement gene cluster in the class III region. The cloning in YACs has been supported by a conventional cosmid walk of 290 kbp in the C4-DRA region. Restriction enzyme sites in the YAC clones were compared to the sites in the cosmid walk, to published cosmid clones, and to the already existing physical maps, leading to a detailed characterization of a region of the human genome over 1500 kbp. The YAC clones will be valuable for functional analysis of the MHC.

引用

页码：3753 / 3757

页数：5

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