SIMPLE INVITRO METHODS FOR TITRATING FELINE IMMUNODEFICIENCY VIRUS (FIV) AND FIV NEUTRALIZING ANTIBODIES

被引：36

作者：

TOZZINI, F

MATTEUCCI, D

BANDECCHI, P

BALDINOTTI, F

POLI, A

PISTELLO, M

SIEBELINK, KHJ

CECCHERININELLI, L

BENDINELLI, M

机构：

[1] UNIV PISA,DEPT BIOMED,VIROL SECT,VIA SAN ZENO 35,I-56127 PISA,ITALY

[2] NATL INST PUBL HLTH & ENVIRONM PROTECT,IMMUNOBIOL LAB,BILTHOVEN,NETHERLANDS

[3] UNIV PISA,DEPT ANIM PATHOL,I-56100 PISA,ITALY

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 37卷 / 03期

关键词：

FELINE IMMUNODEFICIENCY VIRUS; SYNCYTIA FORMATION; VIRUS TITRATION ASSAY; NEUTRALIZATION ASSAY;

D O I：

10.1016/0166-0934(92)90026-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The feline immunodeficiency virus (FIV) readily produced syncytia in Crandell feline kidney (CrFK) cells adapted to a medium containing 0.5% fetal calf serum, a variety of growth factors and other supplements. This finding has been exploited to develop simple and sensitive virus titration and neutralization assays. High titre neutralizing antibodies were detected in cats infected naturally and experimentally with FIV, but not in uninfected animals.

引用

页码：241 / 252

页数：12

共 17 条

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