DISTINCT DNA-BINDING PREFERENCES FOR THE C-MYC MAX AND MAX MAX DIMERS

被引：98

作者：

SOLOMON, DLC ^{[1
]}

AMATI, B ^{[1
]}

LAND, H ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,GROWTH CONTROL & DEV LAB,POB 123,LINCOLNS INN FIELDS,LONDON WC2A 3PX,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 23期

关键词：

D O I：

10.1093/nar/21.23.5372

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcription factor c-Myc and its dimerisation partner Max are members of the basic/helix-loop-helix/leucine-zipper (bHLH-Z) family and bind to the DNA core sequence CACGTG. Using a site-selection protocol, we determined the complete 12 base pair consensus binding sites of c-Myc/Max (RACCACGTGGTY) and Max/Max (RANCACGTGNTY) dimers. We find that the c-Myc/Max dimer fails to bind the core when it is flanked by a 5'T or a 3'A, while the Max/Max dimer readily binds such sequences. Furthermore we show that inappropriate flanking sequences preclude transactivation by c-Myc in vivo. In conclusion, Max/Max dimers are less discriminatory than c-Myc/Max and may regulate other genes in addition to c-Myc/Max targets.

引用

页码：5372 / 5376

页数：5

共 47 条

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