EFFECTS OF NUTRIENTS ON ANTHOCYANIN PRODUCTION IN CULTURED-CELLS OF ARALIA-CORDATA

被引：43

作者：

SAKAMOTO, K ^{[1
]}

IIDA, K ^{[1
]}

SAWAMURA, K ^{[1
]}

HAJIRO, K ^{[1
]}

ASADA, Y ^{[1
]}

YOSHIKAWA, T ^{[1
]}

FURUYA, T ^{[1
]}

机构：

[1] KITASATO INST,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN

来源：

PHYTOCHEMISTRY | 1993年 / 33卷 / 02期

关键词：

ARALIA-CORDATA; ARALIACEAE; CALLUS CULTURE; ANTHOCYANIN; CYANIDIN; 3-XYLOSYLGALACTOSIDE;

D O I：

10.1016/0031-9422(93)85517-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Good results for anthocyanin production in dark-grown calli of Aralia cordata Thunb. were obtained on the addition of sucrose as the carbon source and NH4+ and NO3- at a molar ratio of 1:4 (15 mM total nitrogen). Also, by supplementing NAA as auxin and kinetin as cytokinin, good results were obtained. The anthocyanin yield of high-selected callus cultures was 13.0% maximum dry weight on being cultured for three weeks in darkness. The major anthocyanin, about 70% of the total pigment in the cells, was identified as cyanidin 3-xylosylgalactoside.

引用

页码：357 / 360

页数：4

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