A HIGHLY SENSITIVE METHOD FOR DETERMINING BOTH MN-ZN AND CU-ZN SUPEROXIDE-DISMUTASE ACTIVITIES IN TISSUES AND BLOOD-CELLS

被引：58

作者：

NAKANO, M

KIMURA, H

HARA, M

KUROIWA, M

KATO, M

TOTSUNE, K

YOSHIKAWA, T

机构：

[1] TOHOKU UNIV,DEPT INTERNAL MED 2,SENDAI,MIYAGI 980,JAPAN

[2] KYOTO PREFECTURAL UNIV MED,DEPT INTERNAL MED 1,KAMIGYO KU,KYOTO 602,JAPAN

[3] SMI BRISTOL LTD,SAGAMIHARA,KANAGAWA 229,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 187卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90457-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive method for determining the superoxide dismutase (EC 1.15.1.1) in various tissues and blood cells is described. This method involves inhibition of a cypridina luciferin analog that is chemiluminescence dependent upon O2- generated by hypoxanthinexanthine oxidase. Manganeous superoxide dismutase, which is sensitive to sodium dodecyl sulfate, was determined and calculated by subtraction of superoxide dismutase activity in tissue extract treated with this detergent (CuZn superoxide dismutase) from that in untreated tissue extract (total superoxide dismutase). Both Mn- and CuZn superoxide dismutase activities were expressed as equivalent nanograms of bovine erythrocyte superoxide dismutase per milliliter. Sensitivity limits of the chemiluminescence methods with 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one and 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one as cypridina luciferin analogs were 1 ng and 2-3 ng of superoxide dismutase/ml, respectively. © 1990.

引用

页码：277 / 280

页数：4

共 27 条

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