SEQUENCE-ANALYSIS OF PHOSPHOTYROSINE-CONTAINING PEPTIDES - DETERMINATION OF PTH-PHOSPHOTYROSINE BY CAPILLARY ELECTROPHORESIS

被引：20

作者：

MEYER, HE

HOFFMANNPOSORSKE, E

KORTE, H

DONELLADEANA, A

BRUNATI, AM

PINNA, LA

COULL, J

PERICH, J

VALERIO, RM

JOHNS, RB

机构：

[1] UNIV PADUA,DIPARTIMENTO CHIM BIOL,I-35121 PADUA,ITALY

[2] MILLIGEN BIOSEARCH,BURLINGTON,MA 01803

[3] UNIV MELBOURNE,DEPT ORGAN CHEM,PARKVILLE,VIC 3052,AUSTRALIA

来源：

CHROMATOGRAPHIA | 1990年 / 30卷 / 11-12期

关键词：

CAPILLARY ELECTROPHORESIS; SEQUENCE ANALYSIS; PTH-PHOSPHOTYROSINE; LOCATION OF PHOSPHOTYROSINE;

D O I：

10.1007/BF02269745

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tyrosine phosphorylation is an essential step in the transduction of extracellular stimuli into target cells. Here, we describe a method which allows the localization of phosphotyrosine nonradioactively. Solid phase sequencing of phosphotyrosine-containing peptides and subsequent capillary electrophoresis of PTH-phosphotyrosine permits the unambiguous identification of the phosphoamino acid. Less than l picomol PTH-phosphotyrosine can be determined without any need for radioactive labelling.

引用

页码：691 / 695

页数：5

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