EXPLANT CULTURE OF ADULT GOLDFISH RETINA - MODEL FOR THE STUDY OF CNS REGENERATION

被引：108

作者：

LANDRETH, GE

AGRANOFF, BW

机构：

[1] Neuroscience Laboratory, University of Michigan, Ann Arbor

来源：

BRAIN RESEARCH | 1979年 / 161卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-8993(79)90194-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Conditions are described for culture of retinal explants of adult goldfish which favour outgrowth of neuritic processes onto a substratum. A growth index to quantitate the outgrowth was developed. If the optic nerve is crushed several days prior to explanation, a marked enhancement of neuritic outgrowth is seen relative to control retinas. Histological examination of the explants revealed that retinal ganglion cells in explants from unoperated eyes became hypertrophied in vitro with a time course similar to that observed in vivo following optic nerve crush. Experiments with hemiaxotomized retinas indicate that the perikaryal regenerative response is mediated intracellularly. © 1979.

引用

页码：39 / 53

页数：15

共 49 条

[1] NEURITE OUTGROWTH FROM EXPLANTED XENOPUS RETINA - EFFECT OF PRIOR OPTIC-NERVE SECTION [J].