CLONING AND NUCLEOTIDE-SEQUENCE OF AN ARCHAEBACTERIAL GLUTAMINE-SYNTHETASE GENE - PHYLOGENETIC IMPLICATIONS

被引：27

作者：

SANANGELANTONI, AM ^{[1
]}

BARBARINI, D ^{[1
]}

DIPASQUALE, G ^{[1
]}

CAMMARANO, P ^{[1
]}

TIBONI, O ^{[1
]}

机构：

[1] UNIV LA SAPIENZA, DIPARTIMENTO BIOPATOL UMANA, I-00161 ROME, ITALY

来源：

MOLECULAR AND GENERAL GENETICS | 1990年 / 221卷 / 02期

关键词：

Archaebacteria; Glutamine synthetase; Nuxleotide sequence; Phylogeny;

D O I：

10.1007/BF00261719

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glnA gene of the thermophilic sulphur-dependent archaebacterium Sulfolobus solfataricus was identified by hybridization with the corresponding gene of the cyanobacterium Spirulina platensis and cloned in Escherichia coli. The nucleotide sequence of the 1696 bp DNA fragment containing the structural gene for glutamine synthetase was determined, and the derived amino acid sequence (471 residues) was compared to the sequences of glutamine synthetases from eubacteria and eukaryotes. The homology between the archaebacterial and the eubacterial enzymes is higher (42%-49%) than that found with the eukaryotic counterpart (less than 20%). This was true also when the five most conserved regions, which it is possible to identify in both eubacterial and eukaryotic glutamine synthetases, were analysed. © 1990 Springer-Verlag.

引用

页码：187 / 194

页数：8

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