STEREOSELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF FLURBIPROFEN IN HUMAN PLASMA

被引：53

作者：

GEISSLINGER, G ^{[1
]}

MENZELSOGLOWEK, S ^{[1
]}

SCHUSTER, O ^{[1
]}

BRUNE, K ^{[1
]}

机构：

[1] PAZ,ARZNEIMITTELENTWICKLUNGSGESELLS MBH,W-6230 FRANKFURT 80,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 573卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80492-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enantioselective high-performance liquid chromatographic assay for the quantitation of the enantiomers of flurbiprofen in human plasma is described. The procedure involved extraction of flurbiprofen from acidified plasma into hexane-diethyl ether (8:2, v/v). Stereoselective separation was achieved with a prepacked alpha(1)-acid glycoprotein column without any derivatization procedure. A second assay using a conventional reversed-phase column to determine racemic flurbiprofen is also described. The detection wavelength was set at 246 nm. The limit of quantification was found to be 50 ng/ml for both assays. The method was demonstrated to be sufficiently sensitive for stereoselective pharmacokinetic studies of flurbiprofen.

引用

页码：163 / 167

页数：5

共 17 条

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