INSULIN-LIKE-GROWTH-FACTOR-I (IGF-I) AND IGF-BINDING PROTEINS BLOOD-SERUM LEVELS IN WOMEN WITH EARLY-STAGE AND LATE-STAGE BREAST-CANCER - MUTUAL RELATIONSHIP AND POSSIBLE CORRELATIONS WITH PATIENTS HORMONAL STATUS

被引:33
作者
FAVONI, RE
DECUPIS, A
PERROTTA, A
SFORZINI, S
AMOROSO, D
PENSA, F
MIGLIETTA, L
机构
[1] IST NAZL RIC CANC,DEPT MED ONCOL 2,I-16132 GENOA,ITALY
[2] IST NAZL RIC CANC,DEPT CHEM CARCINOGENESIS,I-16132 GENOA,ITALY
关键词
EARLY AND LATE BREAST CANCER; IGF-I; IGF-BINDING PROTEINS; HORMONES;
D O I
10.1007/BF01218526
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The pathogenesis and progression of breast cancer involve complex interactions between hormones and polypeptide growth factors such as insulin-like growth factor-I (IGF-I). IGF-I has been found in stromal fibroblasts derived from malignant and benign breast tissue and it is a mitogen for several breast cancer cell lines. It circulates bound to specific high-affinity binding proteins, which could act as either positive or negative modulators of tumorigenesis. This, study has been addressed to characterize IGF-I and its binding proteins in the serum of 85 unselected patients with early breast cancer. The IGF-I concentration was assessed by radioimmunoassay of 69 out 85 samples before and after dissociation of the IGF-I and IGF-binding protein (IGF-BP) complex whereas IGF-BP of all 85 sera were analyzed by Western ligand blotting; estradiol and progesterone were measured by radioimmunoassay in native serum samples. In our study no differences in IGF-I serum levels between pre- and post-menopausal patients were observed. Patients with higher estradiol and progesterone serum levels did not present different IGF-I concentrations compared to patients with lower serum levels. Furthermore, IGF-I median values were not found to depend on estrogen receptor (ER) status. A heterogeneous quali-quantitative molecular pattern of binding proteins was detected: IGF-BP3 and IGF-BP1 were the most and the least expressed respectively. No correlations between ER status, or parameters related to the hormonal status, and IGF-I or binding proteins expression were observed. No significant differences in IGF-I concentration and IGF-BP expression were observed between cancer patients and a control group matched for age and menopausal status, Finally, preliminary collection of 20 sera derived from patients with late breast cancer was analyzed for IGF-I and its binding proteins content.
引用
收藏
页码:674 / 682
页数:9
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