METHOD FOR THE EVALUATION OF 3-HYDROXYPROLINE IN THE URINE

被引：18

作者：

SZYMANOVICZ, A

POULIN, G

RANDOUX, A

BOREL, JP

机构：

[1] Laboratoire de Biochimie Médicale, Faculté de Médecine de Reims, F 51095 Reims, 51, rue Cognacq

来源：

CLINICA CHIMICA ACTA | 1979年 / 91卷 / 02期

关键词：

D O I：

10.1016/0009-8981(79)90448-0

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

After hydrolysis of the urine by 6 M HCl, 3-hydroxyproline is purified from many interfering substances by 2 steps of chromatography on Dowex 1 X8 resin equilibrated first in the acetate form and secondly in the OH- form. The amino acid is finally evaluated by chromatography on Dowex 50 M82 resin in a Beckman multichrom amino acid analyzer. In 23 normal adult subjects the mean level was 12.5 ± 3.5 μmol/24 h. In 8 normal children the level was 6.0 ± 5 μmol/24 h and in 8 teenagers 15.2 ± 2.85 μmol/24 h. The ratio of 3-hydroxyproline to 4-hydroxyproline seems indicative of a semiological value of this evaluation in cases of basement membrane collagen deterioration. © 1979.

引用

页码：141 / 146

页数：6