ROLE OF PROTEIN-KINASE-C (PKC) IN BONE-RESORPTION - EFFECT OF THE SPECIFIC PKC INHIBITOR 1-ALKYL-2-METHYLGLYCEROL

被引:14
作者
BOS, MP
MOST, W
VANLEEUWEN, JPTM
HERRMANNERLEE, MPM
机构
[1] LEIDEN UNIV HOSP,DEPT ENDOCRINOL,CLIN INVEST UNIT,2333 AA LEIDEN,NETHERLANDS
[2] ERASMUS UNIV,DEPT INTERNAL MED 3,3000 DR ROTTERDAM,NETHERLANDS
关键词
D O I
10.1016/S0006-291X(05)80026-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The specific inhibitor of protein kinase C, 1-O-alkyl-2-O-methylglycerol (AMG), was studied for its effect on bone resorption, measured as 45Ca-release, in fetal mouse calvariae. AMG (1 to 50 μM) had no effect on basal bone resorption. AMG inhibited parathyroid hormone (40 nM) induced bone resorption in a dose-dependent manner. Resorption induced by 1,25 (OH)2-vitamin D3 (10 nM) or prostaglandin E2 (5 μM) was also inhibited by AMG. The release of β-glucuronidase activity paralled the course of the 45Ca-release. The production of interleukin 6, induced by parathyroid hormone, in fetal rat calvarial osteoblasts was not affected by AMG. AMG (1 to 50 μM) had no cytotoxic effects on cells or calvariae. From these results it is concluded that protein kinase C may have an important role in the regulation of bone resorption. © 1992 Academic Press, Inc.
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页码:1317 / 1323
页数:7
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